青云英语翻译

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翻译结果1翻译结果2 翻译结果3翻译结果4翻译结果5

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要克隆斑马鱼是准备使用ISOGEN试剂(日本基因,东京,日本),然后用无RNA酶的DNA酶(QIAGEN公司,芬洛,荷兰)治疗1个月受精(“强积金”)的斑马鱼β- AR基因,总RNA。使用上标II反转录酶(Invitrogen公司,卡尔斯巴德,美国)进行反转录。 PCR反应用LA Taq酶(大连宝生物,日本滋贺县)。非反转录总RNA被用来作为阴性对照。

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斑马鱼 β-AR 相关基因的克隆,1 个月 postfertilization (强积金) 斑马鱼总 RNA 准备使用 Isogen 试剂日本东京日本基因),然后治疗无核糖核酸酶的核糖核酸荷兰威 QIAGEN)。使用上标二逆转录酶卡尔斯巴德,美国 Invitrogen) 进行逆转录。聚合酶链反应的反应进行了使用拉 Taq (宝生物、 滋贺县、 日本)。非反向转录的 RNA 被用作一个负面的控件。

翻译结果3复制译文编辑译文朗读译文返回顶部

斑马鱼 β-AR 相关基因的克隆,1 个月 postfertilization (强积金) 斑马鱼总 RNA 准备使用 Isogen 试剂日本东京日本基因),然后治疗无核糖核酸酶的核糖核酸荷兰威 QIAGEN)。使用上标二逆转录酶卡尔斯巴德,美国 Invitrogen) 进行逆转录。聚合酶链反应的反应进行了使用拉 Taq (宝生物、 滋贺县、 日本)。非反向转录的 RNA 被用作一个负面的控件。

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正在翻译,请等待...

翻译结果5复制译文编辑译文朗读译文返回顶部

要克隆zebrafish β-AR基因,总RNA从一个月的postfertilization (mpf) zebrafish使用Isogen试剂(日本基因,东京,日本)准备然后对待了与无核糖核酸酶脱氧核糖核酸酶(QIAGEN, Venlo,荷兰)。 反向副本使用上标II反向Transcriptase (Invitrogen, Carlsbad,美国)执行了。 PCR反应使用LA Taq (TaKaRa生物, Shiga,日本)执行了。 非反向被抄录的总RNA使用了作为消极控制。
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